Application

Immunohistochemistry Analysis: 1:1500 dilution from a previous lot detected InsP3R in purkinje cells at the junction of the granular and molecular layers of normal rat cerebellum tissue.

Immunoprecipitation Analysis: 10 µg of Anti-InsP3R, Type 1 immunoprecipitated InsP3R, Type 1 from rat brain microsomal prep RIPA lysate.

This Anti-InsP3R Antibody, Type 1 is validated for use in WB, IP, IH(P) for the detection of InsP3R.

General description

The inositol (1,4,5)-trisphosphate receptor (InsP3R) regulates calcium release from storage within cells and plays an important role in neuronal function. InsP3R has been linked to Huntington's disease. InsP3R has also been linked to the association between cAMP and Ca2+ signaling in regions of the brain, including the neostriatum.

Immunogen

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of rat InsP3R.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in rat brain microsomal tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected InsP3R on 10 µg of rat brain microsomal tissue lysate.

Specificity

This antibody recognizes InsP3R at the cytoplasmic domain.

Target description

~300 kDa observed. UniProtKB/Swiss-Prot P29994 (ITPR1_RAT) entry describes 8 isoforms produced by alternative splicing: Isoform 1 at 313.264 kDa, Isoform 2 at 311.498 kDa, Isoform 3 at 313.136 kDa, Isoform 4 at 311.370 kDa, Isoform 5 at 311.210 kDa, Isoform 6 at 309.444 kDa, Isoform 7 at 308.674 kDa, and Isoform 8 at 306.909 kDa